Methacholine Challenge Test Kit


methacholine challenge test 600 2


The Edge Methacholine Challenge Test Kit comes as a pre-filled ready-to-administer set of 5 sterile solutions. Now with room temperature storage and extended beyond use dating.

Reduce Errors

  • Prefilled, color coded, and barcoded unit dose syringes are simple, safe, and convenient to use.
  • No compounding or dilutions are necessary in the Pharmacy or by a respiratory therapist because our kit comes ready-to-administer.
  • No more mislabeled or unlabeled syringes.

Reduce Risks

  • Every batch of our Methacholine Challenge Kits undergoes rigorous testing to verify the contents of each syringe for sterility, potency, and endotoxin limits.

Reduce Waste

  • Stability studies have allowed us to provide extended Beyond Use Dating (BUD) and room temperature storage, virtually eliminating wasted doses.

Methacholine Challenge Test Kit Concentrations (3 mL per Syringe)

  • 16 mg/ml
  • 4 mg/ml
  • 1 mg/ml
  • 0.25 mg/ml
  • 0.0625 mg/ml


Literature Review


Methacholine Challenge Test (MCT)

The diagnosis of asthma is based on a number of factors, including a patient’s overall health, a detailed medical history, a physical exam, symptoms, and test results.(1) Historically, asthma is often difficult to diagnose because of the non-specific symptoms that characterize the disease.(2) A physician may conduct a challenge study to measure lung function in order to diagnose asthma. Challenge studies may be referred to by a number of different names, including: Methacholine Challenge Test (MCT), Methacholine Inhalation Test, Bronchoprovocation Test, Histamine Challenge Test, and Histamine Inhalation Test.(3)

With the MCT, a physician attempts to trigger asthma symptoms by having the patient inhale the substance methacholine, which causes the airways to narrow in people with asthma.(1) After a spirometry test to determine baseline lung function, the patient is asked to breathe in a low dose of methacholine. The amount of the methacholine is gradually increased. When the lungs show a significant worsening in function (20 percent decrease in breathing ability) the test is complete and indicates a diagnosis of asthma.(3)

One of the recommended methacholine dosing schedules, published in the American Journal of Respiratory and Critical Care Medicine, is as follows: (100-mg vial of methacholine chloride and five-breath dosimeter protocol) E. 0.0625 mg/ml, D. 0.25 mg/ml, C. 1 mg/ml, B. 4 mg/ml, A. 16mg/ml.(4)

Methacholine challenge testing is most often considered when asthma is a serious possibility and traditional methods, most notably spirometry, have not been conclusive.4 “Symptoms that suggest asthma include wheezing, dyspnea, chest tightness, or cough in the following circumstances: (1) with exposure to cold air, (2) after exercise, (3) during respiratory infections, (4) following inhalant exposures in the workplace, and (5) after exposure to allergens and other asthma triggers. A history of such symptoms increases the pretest probability of asthma.”(4)

(1) The Mayo Clinic, Asthma: Steps in Testing and Diagnosis,
(2) Sayeedi, I. MD, and Widrich, J. MD, National Center for Biotechnology Information, Methacholine Challenge Test,
(3) American Thoracic Society, Patient Information Series, Lung Function Studies: Methacholine Challenge Test,
(4) American Journal of Respiratory and Critical Care Medicine, Guidelines for Methacholine and Exercise Challenge Testing 1999,


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